| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149259 |
| Chromosome: | chromosome_3 |
| Location: | 2909527 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g162900 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTTGAGACCACCGTATGCTCGAGGGATG |
| Internal bar code: | CGATCGTTCTCGGCATGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 97.9073 |
| LEAP-Seq n confirming: | 3041 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCAGCAAGCCTGAAGAA |
| Suggested primer 2: | AGGTTACCTTGATGTTGCGG |