Insertion junction: LMJ.RY0402.149265_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149265
Chromosome:chromosome 17
Location:4321588
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g730950 KIN2-2,KHP1,FLA10 Kinesin-II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAAAGGGGGCCATCCCCAAGGCGAAGGG
Internal bar code:TCAGGCTGCATGATATCTTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:847
LEAP-Seq percent confirming:98.8532
LEAP-Seq n confirming:1293
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAACGCGACACATACCTT
Suggested primer 2:ACCTCACTCTCCTGCCTTCA