| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149279 |
| Chromosome: | chromosome 2 |
| Location: | 6271827 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114400 | LIPA2,LAS2 | (1 of 1) PTHR10949//PTHR10949:SF12 - LIPOYL SYNTHASE // LIPOYL SYNTHASE, CHLOROPLASTIC; Lipoic acid synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGCGCCATGCCCGGCGCGACGTGCCA |
| Internal bar code: | ATTTCCGCGGCTTGGGTCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 98.8142 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTGCACCTACGCTGAC |
| Suggested primer 2: | CTTTTGCTCCTACTGCAGGG |