| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149298 |
| Chromosome: | chromosome 6 |
| Location: | 5361452 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGCCGATGTCCTCCTACCGTTGCTAC |
| Internal bar code: | TTCCTAACGTCAAGCTACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 99.3694 |
| LEAP-Seq n confirming: | 1891 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCATTATGCACGCCAAC |
| Suggested primer 2: | GCATATACACGTCACACCGC |