Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.149302 |
Chromosome: | chromosome 5 |
Location: | 1464833 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g243900 | (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTGGGTCCTAGATGACTGGGGAGGGG |
Internal bar code: | GGGTGTCGCGGGTTCGGAGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 640 |
LEAP-Seq percent confirming: | 98.3666 |
LEAP-Seq n confirming: | 1084 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTGTCTGCGCACGTGTAT |
Suggested primer 2: | GTCTGAAATGTTGGGTGGCT |