Insertion junction: LMJ.RY0402.149302_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.149302
Chromosome:chromosome 5
Location:1464845
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g243900 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTCCGCTCATGCGCATCACCCCCCCAC
Internal bar code:TTAGGTTGTTGCGTGCCCCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.743648
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:4805
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGTCTGCGCACGTGTAT
Suggested primer 2:GTCTGAAATGTTGGGTGGCT