| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149317 |
| Chromosome: | chromosome 7 |
| Location: | 4331866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341350 | TRP11 | Transient receptor potential ion channel protein; (1 of 6) PTHR10582 - TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTGACATTATCTCGCCTCAGAGCTCGC |
| Internal bar code: | GGCGCAATAGGACCAAGACTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 99.5798 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTCCAGAATGTCAGGGT |
| Suggested primer 2: | AGAGGGTATGGAGCTTGGGT |