| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149326 |
| Chromosome: | chromosome_2 |
| Location: | 611911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077850 | FAP212 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCAGCCGCATATACTTGAGGCGGGTAGAA |
| Internal bar code: | CGAGCTGCTATCACGTAGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCAAACACCCATTTTT |
| Suggested primer 2: | CGAACTCCACACCCAGAAAT |