Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.149327 |
Chromosome: | chromosome 3 |
Location: | 8129070 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g200750 | DMC1 | Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTGTCCCGCTTGGAACCTTGCACTTG |
Internal bar code: | CGGTCATTCCAATCCAACGATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 463 |
LEAP-Seq percent confirming: | 99.6053 |
LEAP-Seq n confirming: | 5804 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACACACACACACGCACAC |
Suggested primer 2: | TAGTGGACGACTCAGATGCG |