Insertion junction: LMJ.RY0402.149327_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.149327
Chromosome:chromosome 3
Location:8129070
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g200750 DMC1 Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTTGTCCCGCTTGGAACCTTGCACTTG
Internal bar code:CGGTCATTCCAATCCAACGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:99.6053
LEAP-Seq n confirming:5804
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACGCACAC
Suggested primer 2:TAGTGGACGACTCAGATGCG