| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149329 |
| Chromosome: | chromosome 16 |
| Location: | 646635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691800 | FLVB1,FLVB | (1 of 2) 1.5.3.22 - Coenzyme F420H(2) oxidase; Thylakoid flavodiiron protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGACGTGGCGCCTGTTGACCACTCGAG |
| Internal bar code: | TATCTAGACGGACCGGCTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1360 |
| LEAP-Seq percent confirming: | 85.7249 |
| LEAP-Seq n confirming: | 1153 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCACCTGTAACTAGCCC |
| Suggested primer 2: | TGGATTTGACCCTGTTAGCC |