| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149343 |
| Chromosome: | chromosome 10 |
| Location: | 1857463 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431350 | TGL16 | (1 of 4) PTHR12406//PTHR12406:SF27 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED // SUBFAMILY NOT NAMED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTAAGCAAAACGTCCGAGACATGTTGT |
| Internal bar code: | AGGGACGGTAGTTACAACCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.6845 |
| LEAP-Seq n confirming: | 3475 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAAGGCCTATGGGTTCG |
| Suggested primer 2: | ACATGGCCACCTTTCTTGTC |