| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149379 |
| Chromosome: | chromosome 17 |
| Location: | 1676453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708450 | MMP33 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATTCATCTTTTCGCACGCCCCCCCCC |
| Internal bar code: | TCCAGAAGTGTTGATGGCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 99.7647 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCAGTTCTAGTCAGGGG |
| Suggested primer 2: | GCCATTTGCCAATTGTTTCT |