| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149382 |
| Chromosome: | chromosome 14 |
| Location: | 3216415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629200 | FAP98 | Flagellar Associated Protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAACTGGAGGTCAGGGGTTCCCCTGTGT |
| Internal bar code: | CACGGGATCGTGGCAGTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 98.2409 |
| LEAP-Seq n confirming: | 1843 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCAACCCATCAGGAAC |
| Suggested primer 2: | GCATCTGAAGGACTGTGCAA |