| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149392 |
| Chromosome: | chromosome 8 |
| Location: | 4626889 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383450 | FIST N domain protein; (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGGAAGTGGGTGGAGTTTTGAGGGGGGC |
| Internal bar code: | AAGCGTAAATCCCATTTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.5951 |
| LEAP-Seq n confirming: | 492 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTCCAGCAAACCCTTC |
| Suggested primer 2: | CAGCCCCTCCAGTATGTTGT |