Insertion junction: LMJ.RY0402.149398_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.149398
Chromosome:chromosome 16
Location:1789575
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g655200 PTB6 Sodium/phosphate symporter PTB6b; (1 of 9) K14640 - solute carrier family 20 (sodium-dependent phosphate transporter) (SLC20A, PIT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCGCCGGCTGCGTGGCTCTGGACAAGC
Internal bar code:GGCACTGAGTCATCCACACCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:99.8255
LEAP-Seq n confirming:572
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTCTTCATTAACGGTGC
Suggested primer 2:TGGAGCTGTCTGACTTGTGG