Insertion junction: LMJ.RY0402.149411_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.149411
Chromosome:chromosome 10
Location:4841786
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g454200 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTGCACTAGAAAGCGCGAGGAAGCGAGA
Internal bar code:ACCGCACTTGGTTTGATCTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:98.5294
LEAP-Seq n confirming:402
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGAGCGCTACGGTATG
Suggested primer 2:ATGTATAAGGCCGACAACGC