| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149424 |
| Chromosome: | chromosome_11 |
| Location: | 2702524 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476850 | DRC4,PF2 | Component of dynein regulatory complex | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGGAATGGGGATGGGTGCCGGGGATGCA |
| Internal bar code: | CGGCCGGCCTCAATGATTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 92.1518 |
| LEAP-Seq n confirming: | 3229 |
| LEAP-Seq n nonconfirming: | 275 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGACACACATATCCACGG |
| Suggested primer 2: | GAAGGCAGTTGAGGACAAGC |