| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149426 |
| Chromosome: | chromosome 6 |
| Location: | 7836348 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTTGCTCGCGGGCTTTGCGCACAGTCT |
| Internal bar code: | TCCTGATTTGGGAGACGCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 95.8281 |
| LEAP-Seq n confirming: | 758 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTTGGGCATTTGGTGT |
| Suggested primer 2: | GTCTCCGTGAGGTCCTTGAC |