| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149428 |
| Chromosome: | chromosome 7 |
| Location: | 4150496 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342550 | SMM28 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108:SF827 - METHYLTRANSFERASE-LIKE PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGGCTGAAACCAAAATAAGGAGACAA |
| Internal bar code: | CAGACTAACAGGGTGGGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 98.1583 |
| LEAP-Seq n confirming: | 30912 |
| LEAP-Seq n nonconfirming: | 580 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGGAAATCGTCATCAT |
| Suggested primer 2: | TACTGCCGCTGCTACTGCTA |