| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149441 |
| Chromosome: | chromosome 7 |
| Location: | 3340486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334900 | (1 of 3) IPR004089 - Methyl-accepting chemotaxis protein (MCP) signalling domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGGGGTGCGATGGGGTGGGTCACGTGT |
| Internal bar code: | TCCCAGTCCCGAATCCCTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 94.3458 |
| LEAP-Seq n confirming: | 1435 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAAGCACAGGATGTGT |
| Suggested primer 2: | GCCACCATAAGCTTCTCAGC |