Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.149444 |
Chromosome: | chromosome 17 |
Location: | 5131920 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g735200 | KIN1-2,KIN1B | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACGAACACAGTGGGGGAAGGGAGGCGC |
Internal bar code: | GAGCACGGCTCCGGCTACGTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 162 |
LEAP-Seq percent confirming: | 98.4556 |
LEAP-Seq n confirming: | 255 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCAGACACACACACACACA |
Suggested primer 2: | GCAACTGCACTAGCCATCAA |