| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149496 |
| Chromosome: | chromosome 17 |
| Location: | 5554421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737525 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGGCGTGGGGTGTGGGGGCCGGTGGC |
| Internal bar code: | GGTTAATGCGAGATCGGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 96.9416 |
| LEAP-Seq n confirming: | 1046 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAATGCTCGATACGTGA |
| Suggested primer 2: | GGTATCTGCTAAAGCGCCAG |