| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149500 |
| Chromosome: | chromosome 12 |
| Location: | 8515063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547800 | SNE15 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; NAD dependent epimerase/dehydratase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCCACCAGCGGCCAGCATTGTGTCAG |
| Internal bar code: | TCGTAAGTCGTTTAGTTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 99.4755 |
| LEAP-Seq n confirming: | 6828 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCGTTCCTGAAATCGT |
| Suggested primer 2: | CACTACCCCGACCACACTCT |