| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149519 |
| Chromosome: | chromosome 13 |
| Location: | 676159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566300 | RRM12,RSMD | (1 of 1) 2.1.1.171 - 16S rRNA (guanine(966)-N(2))-methyltransferase / M(2)G966 methyltransferase; Putative rRNA (guanine-N2-)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCAGTCCTTCGTAAGATGGCTGCTGC |
| Internal bar code: | GAGCAATAGGACGTGTCAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 64.5563 |
| LEAP-Seq n confirming: | 3579 |
| LEAP-Seq n nonconfirming: | 1965 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTTATACCACCAGGCGT |
| Suggested primer 2: | TTGGTCTTGTCTTGGGTTCC |