| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149520 |
| Chromosome: | chromosome 7 |
| Location: | 5692134 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATAAACGCAAAGGCGTACGGCCCACTGT |
| Internal bar code: | GGGTGGGGATAAGACCCATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 90.9756 |
| LEAP-Seq n confirming: | 746 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGAAGTGCCGTAAGTA |
| Suggested primer 2: | CCATAATGACGCCAACAGC |