| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149548 |
| Chromosome: | chromosome 17 |
| Location: | 4564321 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732802 | (1 of 1) 3.7.1.2 - Fumarylacetoacetase / Fumarylacetoacetate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGCTGCAGGCGTCGCGGCTGGCAGCG |
| Internal bar code: | GTCTGGGGCAGCCTCGGACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 97.8506 |
| LEAP-Seq n confirming: | 9651 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACGTGAGCGGATATGGG |
| Suggested primer 2: | CAGGTTCTTGAGGTTGGAGC |