| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149548 |
| Chromosome: | chromosome 17 |
| Location: | 4564328 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732802 | (1 of 1) 3.7.1.2 - Fumarylacetoacetase / Fumarylacetoacetate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCCCGGACCGCTCCTCCCGCCGCTCC |
| Internal bar code: | TTGTTGACGCCTTTTGCGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTCTTGAGGTTGGAGC |
| Suggested primer 2: | AGTACGTGAGCGGATATGGG |