Insertion junction: LMJ.RY0402.149558_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.149558
Chromosome:chromosome 9
Location:2803394
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g388900 TEH10 Oleoyl-[acyl-carrier-protein] thioesterase/hydrolase; (1 of 1) PTHR11487//PTHR11487:SF0 - THIOESTERASE // S-ACYL FATTY ACID SYNTHASE THIOESTERASE, MEDIUM CHAIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTTGTGTATGCATAACCCCTGAGCATG
Internal bar code:GGGCCTTGGGACGCACAGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:903
LEAP-Seq percent confirming:98.836
LEAP-Seq n confirming:1953
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGCAAGGCTTGTTCTC
Suggested primer 2:TAGGATCTTGCACCACCTCC