| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149560 |
| Chromosome: | chromosome 13 |
| Location: | 3594961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588350 | (1 of 1) IPR000403//IPR011009 - Phosphatidylinositol 3-/4-kinase, catalytic domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTTTTGCTAGCCCGTCCGCCCCGCCCT |
| Internal bar code: | AGGACTCGTCCCGGCTCTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCGCAAGTTAAACGAT |
| Suggested primer 2: | CAAACACATGCCAACCAGTC |