| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149574 |
| Chromosome: | chromosome 12 |
| Location: | 7121286 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGCTAACGGCGCCGCCGCCTGCGGG |
| Internal bar code: | GTGCCCGTATAAAAGCGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 718 |
| LEAP-Seq percent confirming: | 41.8215 |
| LEAP-Seq n confirming: | 1148 |
| LEAP-Seq n nonconfirming: | 1597 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCACCAAAAGACACTGA |
| Suggested primer 2: | ACACACACACACACACGCAC |