Insertion junction: LMJ.RY0402.149575_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.149575
Chromosome:chromosome 15
Location:1339687
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g641950 (1 of 1) PF14846 - Domain of unknown function (DUF4485) (DUF4485) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCTTACTTGCCACTCCGGGGGTCCCTAA
Internal bar code:GATAGACAAGAGGTGATAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:17.6471
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTCAACAGCCTCCGTTTC
Suggested primer 2:AAGTCGTACGGCTCAACAGG