| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149575 |
| Chromosome: | chromosome 15 |
| Location: | 1339695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641950 | (1 of 1) PF14846 - Domain of unknown function (DUF4485) (DUF4485) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACGATCACGATCACGTATGGCTTCTGT |
| Internal bar code: | TGTGGGCGAAGGCGGGCAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCAACAGCCTCCGTTTC |
| Suggested primer 2: | AAGTCGTACGGCTCAACAGG |