Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.149620 |
Chromosome: | chromosome_4 |
Location: | 2002961 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g218800 | THB3 | Truncated hemoglobin | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACCGTAGATGGGCGTGTCGTGTGCGCAGC |
Internal bar code: | TATCCTACACTTTTTTACTGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 334 |
LEAP-Seq percent confirming: | 98.4012 |
LEAP-Seq n confirming: | 8555 |
LEAP-Seq n nonconfirming: | 139 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAATCGCACAGCTTATTGC |
Suggested primer 2: | ACTGACACGCGTCATCTCAC |