| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149633 |
| Chromosome: | chromosome 3 |
| Location: | 3852963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170850 | MMP19 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCGGGCGATTCGCTCCACATCCGGTT |
| Internal bar code: | TGGGGATCATTTTACTGCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.6283 |
| LEAP-Seq n confirming: | 11793 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCAAGCCGTTCCTCTA |
| Suggested primer 2: | TGCTTTGAACATGGCTCTTG |