Insertion junction: LMJ.RY0402.149646_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149646
Chromosome:chromosome 2
Location:2717239
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGATCTAGGTGCAGGGACGGGCAACGC
Internal bar code:GGGGACTGCTGCGAAACGCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:99.3108
LEAP-Seq n confirming:8358
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:AACCAATGCTGACCTTTTCG
Suggested primer 2:CACAAACAGCTGCTAACCGA