Insertion junction: LMJ.RY0402.149652_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149652
Chromosome:chromosome 10
Location:4013333
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g449050 (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACAGGGGGCGGGGGCCGGGGCTCGGGGC
Internal bar code:GATGCCGTGGCGGGACGGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:149
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGTACGTCAAGTCGGG
Suggested primer 2:ATGGGACTAGACACGTTGCC