| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149692 |
| Chromosome: | chromosome 12 |
| Location: | 4472384 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAACAGGGCCTGGGCCCGATCCACGTA |
| Internal bar code: | TGCCCATGGCAGATCCCACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 98.8784 |
| LEAP-Seq n confirming: | 4408 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGATGTGTGGAGAAAGAG |
| Suggested primer 2: | CTGTTCAGGTTCCCTTGCAT |