| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149692 |
| Chromosome: | chromosome 15 |
| Location: | 774501 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640101 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCACCTCGTCGCTGCGGGGCCGACCCAC |
| Internal bar code: | TAGTGGACTCGGATCGGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1105 |
| LEAP-Seq percent confirming: | 87.6991 |
| LEAP-Seq n confirming: | 991 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGGGTGTGATTGTGTG |
| Suggested primer 2: | AACCGAACCTAACCAACACG |