| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149711 |
| Chromosome: | chromosome 16 |
| Location: | 6757651 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673393 | (1 of 2) PTHR13710//PTHR13710:SF81 - DNA HELICASE RECQ FAMILY MEMBER // ATP-DEPENDENT DNA HELICASE Q-LIKE 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCGCAGGGTGCTGCCGCACCTGGCAA |
| Internal bar code: | GTGATAATAGTCCGGGATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 99.7468 |
| LEAP-Seq n confirming: | 788 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAGCTAGGATGGTGAG |
| Suggested primer 2: | AGCCAGAACCACAAATGACC |