| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149713 |
| Chromosome: | chromosome 1 |
| Location: | 332681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001983 | C2-domain containing protein; (1 of 2) PTHR32246//PTHR32246:SF18 - FAMILY NOT NAMED // C2 DOMAIN-CONTAINING PROTEIN-RELATED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGGGCCGGATGCGCTTCGCCGAGGCGC |
| Internal bar code: | TTGCTCAGCTTCTAGGGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 98.1931 |
| LEAP-Seq n confirming: | 3478 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCCGTTGTCGTCTATT |
| Suggested primer 2: | CCCCACCCCCTTCTTAAATA |