| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149722 |
| Chromosome: | chromosome 11 |
| Location: | 1284791 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467708 | (1 of 1) IPR029022 - ABC transporter, BtuC-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGTCCTGTCCCCCTCCGACTCCACGCT |
| Internal bar code: | CTATACAGCGGGGCGGTACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 58.5377 |
| LEAP-Seq n confirming: | 7710 |
| LEAP-Seq n nonconfirming: | 5461 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTTCGTGAAGTTCGAG |
| Suggested primer 2: | TCGTTCCTCTCTTCCCTCAA |