| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149722 |
| Chromosome: | chromosome 11 |
| Location: | 1284794 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467708 | (1 of 1) IPR029022 - ABC transporter, BtuC-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGCCCTGGGCCCTGGCTTGACGTACA |
| Internal bar code: | GTGATGCACCGAGTGAAAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 96.6062 |
| LEAP-Seq n confirming: | 12895 |
| LEAP-Seq n nonconfirming: | 453 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCCTCTCTTCCCTCAA |
| Suggested primer 2: | TGACGTTCGTGAAGTTCGAG |