Insertion junction: LMJ.RY0402.149726_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.149726
Chromosome:chromosome 12
Location:6140125
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g536050 ALA1 (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); Phospholipid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTATGGCGTCGACAACAGTCGCCGCGTCG
Internal bar code:GCGATGTGTTGGTTGCTAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:297
LEAP-Seq percent confirming:7.20721
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:103
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCAGAAGAACATGAGGA
Suggested primer 2:CACCTGCTACATCGAGACGA