| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149738 |
| Chromosome: | chromosome 13 |
| Location: | 5159934 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACTGGAGTTGGCTGGCAGGATTGAA |
| Internal bar code: | CCTTGGCTTGGTACAGACCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 98.9219 |
| LEAP-Seq n confirming: | 2661 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAGGGGTAGGAATTGTT |
| Suggested primer 2: | AAACGTTGTGGGGCTCTATG |