| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149739 |
| Chromosome: | chromosome 9 |
| Location: | 932600 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401050 | (1 of 12) 2.7.11.18 - [Myosin light-chain] kinase / Smooth-muscle-myosin-light-chain kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTACGGCAGACCGCGCGGCGTGGTTGG |
| Internal bar code: | GTAGCGCTAGAGGAAGGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.325 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTCGTATGGGGTTAGG |
| Suggested primer 2: | GATAGCAGCGGGGTATGTGT |