| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149751 |
| Chromosome: | chromosome 16 |
| Location: | 3126409 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665650 | HFLX1,HFLX | Putative chloroplast GTP-bidning protein involved in ribosome hibernation or ribosome biogenesis; (1 of 1) K03665 - GTP-binding protein HflX (hflX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCGCAAACCCAACCGCTGCCTCCCAA |
| Internal bar code: | CTCACTCCGTGGCTTGACAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 370 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATAAGCGAGGCACAGAA |
| Suggested primer 2: | TGTTGCACATACGTTTCGGT |