Insertion junction: LMJ.RY0402.149770_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149770
Chromosome:chromosome 14
Location:3826954
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCTCATCAAGACATCTCGAGCACTGGC
Internal bar code:CCGCAACCGGTAACCAGCTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:708
LEAP-Seq percent confirming:54.6031
LEAP-Seq n confirming:16631
LEAP-Seq n nonconfirming:13827
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGCATAGGCTACACTCC
Suggested primer 2:CACACACACACAAGCACCTG