| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149774 |
| Chromosome: | chromosome 12 |
| Location: | 8528927 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547700 | TTL14 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL); Putative tubulin tyrosine ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACCCTGCAATGCCCCTGACATGCCAC |
| Internal bar code: | TTGTTTGACTTATACGTACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGAAACTGCCAATGGAC |
| Suggested primer 2: | ACAGCAGGGAACTGGTATGG |