| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149781 |
| Chromosome: | chromosome 9 |
| Location: | 2992488 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387550 | CGL142 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCGCCACGCCCCAGTTGTACAGAGCCG |
| Internal bar code: | GGGTGTTCAGGGCGTGACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.6183 |
| LEAP-Seq n confirming: | 2088 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATGAAGGAGAAGTCACA |
| Suggested primer 2: | CATCAGGCTGAGCTGTGGTA |