| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149790 |
| Chromosome: | chromosome 15 |
| Location: | 384585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636004 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTCGCTCCCCGAGTACGGCTCCAGCTC |
| Internal bar code: | CCAGTAATATTTGTGTCGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1233 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 956 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTTTGGAAAAGCGGTA |
| Suggested primer 2: | CCTCTTGACTACTCCGACGC |